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addgene plasmid repository  (Addgene inc)


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    Addgene inc addgene plasmid repository
    Addgene Plasmid Repository, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/addgene plasmid repository/product/Addgene inc
    Average 93 stars, based on 37 article reviews
    addgene plasmid repository - by Bioz Stars, 2026-03
    93/100 stars

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    In untreated mitochondria, ATP synthases were found in high abundance on cristae membranes and rarely on the IBM (A and inset). After OA treatment, the OM-associated class of ATP synthases significantly increased in population (B and insets). Segmentation of the mitochondrial membrane with ATP synthase molecules identified by Pytom back projected onto cristae (cyan spheres) and the OM (magenta spheres) for visualization. Quantification of ATP synthases from each class in untreated (n=17 IBM, n=623 cristae) and OA treated cells (n=257 IBM, n=413 cristae) and comparison via Fisher’s exact test with template match density map shown and model docked (RCSB: 8H9T) (C). Live cell Airyscan imaging of mitochondria using <t>TOMM20-mCherry</t> and ATP5F1B-GFP to track ATP synthase localization after depolarization. (D) Quantification of localizations in (C) (n=3-4 cells per replicate over three independent replicates with a Paired t test used to determine significance).
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    In untreated mitochondria, ATP synthases were found in high abundance on cristae membranes and rarely on the IBM (A and inset). After OA treatment, the OM-associated class of ATP synthases significantly increased in population (B and insets). Segmentation of the mitochondrial membrane with ATP synthase molecules identified by Pytom back projected onto cristae (cyan spheres) and the OM (magenta spheres) for visualization. Quantification of ATP synthases from each class in untreated (n=17 IBM, n=623 cristae) and OA treated cells (n=257 IBM, n=413 cristae) and comparison via Fisher’s exact test with template match density map shown and model docked (RCSB: 8H9T) (C). Live cell Airyscan imaging of mitochondria using TOMM20-mCherry and ATP5F1B-GFP to track ATP synthase localization after depolarization. (D) Quantification of localizations in (C) (n=3-4 cells per replicate over three independent replicates with a Paired t test used to determine significance).

    Journal: bioRxiv

    Article Title: In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment

    doi: 10.1101/2025.03.24.645001

    Figure Lengend Snippet: In untreated mitochondria, ATP synthases were found in high abundance on cristae membranes and rarely on the IBM (A and inset). After OA treatment, the OM-associated class of ATP synthases significantly increased in population (B and insets). Segmentation of the mitochondrial membrane with ATP synthase molecules identified by Pytom back projected onto cristae (cyan spheres) and the OM (magenta spheres) for visualization. Quantification of ATP synthases from each class in untreated (n=17 IBM, n=623 cristae) and OA treated cells (n=257 IBM, n=413 cristae) and comparison via Fisher’s exact test with template match density map shown and model docked (RCSB: 8H9T) (C). Live cell Airyscan imaging of mitochondria using TOMM20-mCherry and ATP5F1B-GFP to track ATP synthase localization after depolarization. (D) Quantification of localizations in (C) (n=3-4 cells per replicate over three independent replicates with a Paired t test used to determine significance).

    Article Snippet: Plasmid transfection occurred in Opti-MEM™ I Reduced Serum Medium (Thermo Fisher Scientific, REF: 31985-070) with Lipofectamine 2000 reagent (Thermo Fisher Scientific, REF:11668030). mCherry-TOMM20-N-10 was a gift from Michael Davidson (addgene: 55146; RRID:Addgene_55146).

    Techniques: Membrane, Comparison, Imaging